Microfluidic thrombosis analysis system: possibilities and limitations

Micro and Nano Systems Letters

Table 1 Summary of thrombosis research using microfluidic chips

Control variable	Magnitude of SR [s⁻¹]	Fabrication	Sample	Analysis method	Refs
Hemodynamics
Low SR	< 5000	Photo- & Soft- lithography	Human blood	Fluorescence microscopy Image processing	[43, 46]
Low SR	< 5000	3D printing	Human blood	Culture of ECs Fluorescence microscopy Image processing	[25]
High SR	> = 5000	Photo- & soft- lithography	Human blood	Image processing	[52]
High SR	> = 5000	Micromilling & soft-lithography	Porcine blood	Light transmission detection system Image processing	[5, 33]
Gradient of SR	500–20000	Photo- & Soft- Lithography	Human blood	Fluorescence microscopy Image processing SEM Micro-PIV	[3, 6, 7, 35]
Degree of stenosis	600–29859	Photo- & Soft- Lithography	Human blood	Culture of ECs Fluorescence microscopy Image processing Staining	[18, 22, 32, 44]
Number of stenosis	> = 12,000	Photo- & Soft- Lithography	Human blood	Image processing	[4]
Biochemistry
Kinds of thrombolytic agents	3500	Capillary tube	Porcine blood	Fluorescence microscopy Image processing	[31]
Dose of antiplatelet therapy	500–10000	Micromilling & Soft-lithography	Human blood	Impedance aggregometry	[34]

SR shear rate, ECs endothelial cells, PIV particle image velocimetry, SEM scanning electron microscope