Fig. 2From: Methods to analyze extracellular vesicles at single particle levelPrinciple of optical analysis system for individual EVs. a Nanoparticle tracking analysis (NTA): diameter of particle is predicted according to mean square displacement (MSD) through Brownian motion of individual particles tracked for several frames. b nano-Flow cytometry (nFCM): the particle size is estimated by measuring the intensity of forward-scattered (FSC) and side-scattered (SSC) light in the flow of individual particles focused by the sheath flowBack to article page