Influence of citrate buffer and flash heating in enhancing the sensitivity of ratiometric genosensing of Hepatitis C virus using plasmonic gold nanoparticles

Micro and Nano Systems Letters

Table 1 UV–visible absorbance data (at 520 nm and the ratiometric at 700 nm/520 nm) of Au NPs added to the oligonucleotides hybridized under different conditions before and after salting with two different final concentrations of NaCl. The data obtained are at 95% confidence level

Absorbance	Hybridization conditions	T	C	T—145 mM	C—145 mM	T—340 mM	C—340 mM	T (cit)	C (cit)	T—145 mM (cit)	C—145 mM (cit)	T—340 mM (cit)	C—340 mM (cit)
A₅₂₀	1.5 µM Probe versus Target/Control
	No flash heating	0.43	0.42	0.36	0.25	0.32	0.19	0.42	0.42	0.40	0.24	0.38	0.20
	95 °C—30 s	0.43	0.44	0.38	0.28	0.35	0.21	0.42	0.42	0.42	0.25	0.40	0.19
	95 °C—120 s	0.42	0.41	0.39	0.26	0.35	0.17	0.43	0.41	0.42	0.26	0.39	0.20
	3.0 µM Probe versus Target/Control
	No flash heating	0.40	0.40	0.35	0.34	0.34	0.25	0.40	0.40	0.37	0.33	0.32	0.24
	95 °C—30 s	0.41	0.42	0.35	0.32	0.34	0.24	0.41	0.39	0.38	0.34	0.34	0.22
	95 °C—120 s	0.40	0.39	0.38	0.36	0.35	0.27	0.39	0.39	0.36	0.34	0.35	0.24
A_700/520	1.5 µM Probe versus Target/Control
	No flash heating	0.14	0.13	0.41	0.91	0.53	1.07	0.12	0.12	0.24	0.96	0.37	1.08
	95 °C—30 s	0.13	0.12	0.35	0.79	0.52	1.09	0.12	0.12	0.14	0.96	0.26	1.10
	95 °C—120 s	0.12	0.12	0.33	0.87	0.43	1.03	0.12	0.12	0.14	1.01	0.30	1.11
	3.0 µM Probe versus Target/Control
	No flash heating	0.32	0.36	0.41	0.63	0.45	0.97	0.39	0.29	0.50	0.66	0.51	0.98
	95 °C—30 s	0.40	0.45	0.43	0.56	0.48	0.85	0.15	0.19	0.25	0.53	0.34	1.00
	95 °C—120 s	0.27	0.26	0.36	0.55	0.42	0.91	0.17	0.19	0.28	0.53	0.35	0.98