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Micro and Nano Systems Letters
Fig. 3 | Micro and Nano Systems Letters

Fig. 3

From: A microheater on polyimide substrate for hand-held realtime microfluidic polymerase chain reaction amplification

Fig. 3

a The original DNA amplification PCR fluorescence results for β-actin (amplicon size of 70 bp) genes dying SYBR green 1 before and after 40 amplification cycling in three microchambers si and, b the Agarose gel electrophoresis results for confirmation of amplification after PCR on the chip for β-actin (lanes 1–4) and C. auriculatum (amplicon size of 150 bp, lanes 5–12) while controlling the temperatures and sustain time in steps. Here, M lanes are 25 bp marker, P1 is PCR products to β-actin and P2 is PCR products to Cynanchum auriculatum Royle ex Wight in a commercial thermocycler (UF-150, Genesystem Co., Ltd.) with the same temperature conditions in about 1 h, respectively. c The gel electrophoresis results as a function of starting DNA copies numbers showing the dynamic detection ranges (1.2 × 107 copies, 2.2 × 106 copies, 3.2 × 105 copies, 4.2 × 104 copies, 5.2 × 103 copies, and 6.2 × 102 copies)

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