Fig. 1

Device design and operation mechanism. a Schematic illustration of the particle transfer device and particle arraying device. b Sequential particle arraying mechanism. Incoming particles are sequentially arrayed at each vacant trap site based on flow fractionation. c Single particle trapping by pneumatic microvalve operation. Channel wall is deflected by applied pneumatic pressure and it can trap a single particle. d Once a single particle is trapped, subsequent particles bypass the trap and migrate toward the waste. e Trapped single particle can be released by removal of the applied pneumatic pressure