Fig. 5
From: An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth
Phase-contrast optical images of rat PC12 cells cultured on nanostructured and non-nanostructured microprobes. a At 3 days after NGF treatment, PC12 cells were not attached or differentiated on non-nanostructured microprobes (magnification, ×100). PC12 cells on a nanostructured microprobe b 1-day in vitro culture (DIV1), before the addition of NGF (magnification, ×100), and c 3 days after NGF treatment (magnification, ×200). Cell adhesion and differentiation have been improved for nanostructured microprobes compared to non-nanostructured microprobes. d Number of neurites per differentiated PC12 cell on non-nanostructured and nanostructured microprobes at 5 days after NGF treatment (Student’s t test; mean ± SE, n = 9, ***p < 0.001)