Table 1 Some EV applications in diagnosis
From: Methods to isolate extracellular vesicles for diagnosis
Disease | Biospecimen | Isolation method | Biomarker | Reference |
|---|---|---|---|---|
Breast cancer | Serum | Ultracentrifugation | EpCAM | [54] |
Acute kidney injury (AKI) | Urine | Differential centrifugation | Fetuin-A | [33] |
Ovarian cancer | Serum | Density gradient centrifugation | OVCAR-3 and IGROVI | [55] |
Lung cancer | Plasma | Immunoaffinity | EGFR level | [65] |
Ovarian cancer | Serum | Modified magnetic activated cell sorting procedure | Overexpressed specific miRNAs | [30] |
Prostate cancer | Urine | Ultrafiltration | PCA3 and TMPRSS2-ETG | [53] |
Focal segmental glomerulosclerosis (FSGS) | Urine | Ultrafiltration | Podocalyxin-like protein 1 (PODXL) | [34] |
Ovarian adenoma | Serum | Size exclusion chromatography | EGFRvIII | [77] |
Prostate cancer | Urine | Polymer precipitation | Prostate specific membrane antigen (PSMA) | [85] |
Acute coronary syndromes (ACS) | Serum | Polymer precipitation | Polygenic immunoglobulin receptors, cystatin C(cycteine proteinase) and C5a | [81] |
Ovarian adenoma | Serum | Size exclusion chromatography | EGFRvIII | [77] |
Glioblastoma multiforme (GBM) | Serum | Microfluidics | Isocitrate dehydrogenase (IDH-1) | [90] |
Melanoma cancer | Blood | Microfluidics | MelanA | [33] |