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Table 1 the activation energy of the rate-limiting step of this work is compared with the activation energy reported by prior studies of the reactions related to EET

From: Effect of temperature on a miniaturized microbial fuel cell (MFC)

Reaction

Activation energy (eV)

Rate-limiting step of EET in this work

0.132–0.146

Cytochrome C electron transfer

0.14 ± 0.03 [22, 23]

Quinones reduction

0.477 [24]

Reduction of CoQ by NADH

0.86–1.23 [25]

Ubiquinol oxidation by the bc1 complex

0.466–0.518 [26]

Quinol oxidation

0.466 [27]

Iron-sulfur protein

0.02 [28]

  1. It is found that the activation energy of this work is close to the electron transfer between cytochrome c, suggesting that the rate limiting step is the cytochrome c, especially for a thick biofilm, the activation energy of this work matches very well with the activation energy of cytochrome c electron transfer